利用小种标记分析云南省两个县的小麦条锈菌群体

Population analysis with race-specific-markers of Puccinia striiform isf. sp.tritici from two counties of Yunnan Province

小麦条锈病是由小麦条锈菌（Pucciniastriifor．misf．sp．tritici）引起的气流传播病害，在世界各主要麦区均有发生，也是我国小麦生产上危害最严重的病害之一。小麦条锈菌毒性变异频繁，易产生新毒性小种，导致小麦品种的抗病基因失效，引起小麦条锈病周期性流行。因此，了解小麦种植区的条锈菌群体结构组成及变异，对于制定更为有效的条锈病控制方法具有重要的意义。

In order to understand the population strucutre of wheat stripe rust （Puccinia striiformis f. sp. triti- ci）, five race-specific-markers were used to investigate 251 diseased samples collected from Dali and Yiman, two counties where rust disease occured frequently. The most frequently detected races were CY32 and CY29 in both Dali and Yimen counties, with a detection freqnceny of 82.2% and 51.1% in Dali, and 42.2% and 57.1% in Yimen respectively. The reliability of the results was confirmed by resampling of diseased leaves with the in- fection of race identified by differential varieties method. Several race-combinations were detected in single in- fected leaf. Two or more races infection occured in 44.6% single infected leaves. The results indicated that many leaves were infected by two or more races of the fungus.