辣椒上烟草轻型绿花叶病毒的鉴定

Identification of Tobacco mild green mosaic virus infecting pepper

辣椒源自南美洲，属于茄科辣椒属（CapsicumL．），为重要经济作物。病毒病是影响辣椒生产的主要病害，侵染辣椒的病毒有40余种，

In December 2011, bubbling, mosaic leaves and noticeable chlorosis of fruits were observed on field-grown pepper plants in Xiang＇an district of Xiamen city. To identify the pathogen, six symptomatic plants were tested by ELISA using specific antibodies of Tobacco mild green mosaic virus （TMGMV）, Pepper mild mottle virus （PMMoV） and other viruses infecting pepper. Extracts from all these plants tested positive for TMGMV and PMMoV. To confirm positive results, total RNAs from six symptomatic plants were analyzed by reverse transcription （RT）-PCR with primers designed to specifically amplify the coat protein （CP） gene of TMGMV and PMMoV. The 524 bp TMGMV-CP specific DNA fragment was amplified from all samples and ex- pected PMMoV-CP fragment was amplified from three samples. RT-PCR products of TMGMV-CP were purified and directly sequenced. The obtained CP gene consisted of 480 nucleotides and encodes a putative protein of 159 amino acid residues, sharing identities of 97.9% to 99.8% and 97.5% to 100% at the nucleotide and amino acid level with other TMGMV isolates, respectively. Phylogenetic analysis based on CP nucleotide sequence showed no apparent host or region relationship existed in TMGMV isolates. To our knowledge, this is the first report of TMGMV identified in China.