三种持久性有机污染物对罗非鱼肝脏抗氧化系统的体外影响

In vitro effects of three persistent organic pollutants on antioxidant defense system in Mossambica tilapia liver

以罗非鱼(Mossambica tilapia)为研究对象,研究了海洋环境中三种典型持久性有机污染物[四溴双酚A(TBBPA),多氯联苯(PCB126),荧蒽(Flu)]体外染毒对罗非鱼肝脏抗氧化系统(GSH,SOD,GST)的影响。三种污染物在低浓度(ng/L)时显著抑制GSH含量,高浓度时GSH含量有所回升,浓度超过一定值时,GSH含量迅速下降;GST活性变化趋势与GSH相似,TBBPA 100 ng/L与Flu 50 ng/L暴污时显著诱导GST活性;TBBPA与PCB126仅在最高浓度(100μg/L)时抑制SOD活性,而Flu浓度大于500 ng/L时即显著抑制SOD活性。结果表明,GSH和GST对污染物较为敏感,变化趋势呈现相似性。因此,GSH和GST有可能作为持久性有机污染物早期预警的生物指标。

In vitro effects of tetrabromobisphenol A（TBBPA）,polychlorinated biphenyls（PCB126）,fluoranthene（Flu） on glutathione（GSH） contents,glutathione S-transferase（GST） activity and superoxide dismutase（SOD） activity in Mossambica tilapia liver were examined.Under the experimental doses,GSH contents were inhibited at low level exposure of all the pollutants,then recovered with the increase of concentrations,when the concentration exceeds a certain value,GSH content decreased rapidly;The change of GST activity was similar to GSH,and GST activity was significantly induced exposed to 100 ng/L of TBBPA and 50 ng/L of Flu;SOD activity was significantly inhibited at 100 μg/L exposure of TBBPA and PCB126,when the concentration was greater than 500 ng/L,Flu exposure significantly inhibited SOD activity.The results showed that GSH and GST were sensitive to the pollutants,and their changes were similar.So GSH and GST could be used as a potential biomarker for early warning of the persistent organic pollutants.