加味左金汤对胃溃疡大鼠血清NO及胃黏膜EGF、EGFR表达的影响

Effect of Jiawei Zuojin Decoction on serum NO and Expression of EGF and EGFR of Rats with Gastric Mucosa

目的观察加味左金汤对胃溃疡大鼠血清一氧化氮(NO)及胃黏膜表皮生长因子(EGF)、表皮生长因子受体(EGFR)表达的影响。方法将健康SD大鼠随机分为6组,正常对照组、模型组、加味左金汤大、中、小剂量组与阳性对照组。各组大鼠断尾取血,采用硝酸还原酶法检测胃溃疡大鼠血清NO值;运用免疫组化法检测胃溃疡大鼠胃黏膜EGF、EGFR表达。结果较模型组而言,加味左金汤大、中剂量组能提高胃溃疡大鼠血清NO值(P<0.05),该方大、中、小剂量组均可增加溃疡胃组织EGF和EGFR的阳性细胞表达(P<0.01)。结论加味左金汤能明显提高胃溃疡大鼠血清NO值及胃组织EGF和EGFR的阳性细胞表达。

Objective： To observe effect of Jiawei Zuojin Decoction on serum NO and expression of EGF and EGFR of rats with gastric mueosa. Methods： Blood of rats tail was taken, gastric ulcer rats serum NO value were detected with nitrate reductive enzymatic ;gastric ulcer rat gastric mucosal EGF and EGFR expression were de- tected with immunohistochemieal method. Results： Jiaweizuojintang large, medium close can improve gastric ulcer rats serum NO value;the large, medium and small close group of the party could increase gastric ulcer organization positive cells expression of EGF and EGFR. Conclusion： Jiawei Zuojin Decoction can improve the level of serum NO value and expression of EGF and EGFR of rats with Gastric Mucosa.