摘要
目的观察加味左金汤对胃溃疡大鼠血清一氧化氮(NO)及胃黏膜表皮生长因子(EGF)、表皮生长因子受体(EGFR)表达的影响。方法将健康SD大鼠随机分为6组,正常对照组、模型组、加味左金汤大、中、小剂量组与阳性对照组。各组大鼠断尾取血,采用硝酸还原酶法检测胃溃疡大鼠血清NO值;运用免疫组化法检测胃溃疡大鼠胃黏膜EGF、EGFR表达。结果较模型组而言,加味左金汤大、中剂量组能提高胃溃疡大鼠血清NO值(P<0.05),该方大、中、小剂量组均可增加溃疡胃组织EGF和EGFR的阳性细胞表达(P<0.01)。结论加味左金汤能明显提高胃溃疡大鼠血清NO值及胃组织EGF和EGFR的阳性细胞表达。
Objective: To observe effect of Jiawei Zuojin Decoction on serum NO and expression of EGF and EGFR of rats with gastric mueosa. Methods: Blood of rats tail was taken, gastric ulcer rats serum NO value were detected with nitrate reductive enzymatic ;gastric ulcer rat gastric mucosal EGF and EGFR expression were de- tected with immunohistochemieal method. Results: Jiaweizuojintang large, medium close can improve gastric ulcer rats serum NO value;the large, medium and small close group of the party could increase gastric ulcer organization positive cells expression of EGF and EGFR. Conclusion: Jiawei Zuojin Decoction can improve the level of serum NO value and expression of EGF and EGFR of rats with Gastric Mucosa.
出处
《中国中医急症》
2013年第11期1836-1838,共3页
Journal of Emergency in Traditional Chinese Medicine
基金
陕西省中医管理局科研课题(JC68)
陕西省教育厅科研课题(11JK0681)
陕西中医学院重点科研项目(2009XJ-05)