高效液相色谱法测定乐脉片中芍药苷含量

Determination of Paeoniflorin in Lemai Tablets by HPLC

目的建立测定乐脉片中芍药苷含量的高效液相色谱法。方法色谱柱为Waters Symmetry Shield RPl8柱（150mm×3．9mm，5μm），流动相为乙腈-0．05％磷酸（13：87），检测波长230nm。结果芍药苷进样量在0．20403—4．08060μg范围内与峰面积呈良好的线性关系，r=0．9995。平均回收率为100．52％，RSD=2．03％（n=6）。结论该方法简便、快速准确、灵敏度高、重现性好，可作为乐脉片中芍药苷的含量测定法。

Objective To establish a method for the determination of the paeonifiorin content in Lemai Tablet by HPLC. Methods The HPLC system consisted of the Waters SymmetryShield RP18 column（150 mm x3.9 mm,5 μm） with acetonitrile- 0.05% phos- phoric acid（ 13：87） as the mobile phase,the detection wavelength was set at 230 nm. Results The sample size of paeoniflorin in the range of 0.204 03-4.080 60 μg had the good linear relation with the peak area. The average recovery rate was 100.52% （n=6, RSD = 2.03% ）. Conclusion This method is convenient, quick and accurate with hith sensitivity an dgood reproducibility,which is suit- able for the determination of the paeoniflorin content in Lemai Tablet.