荧光法对胸水肿瘤细胞的鉴别诊断

Differentiated diagnosis of tumor cells in thoracic liquid by fluorescent method

目的 :探讨荧光法检测胸水肿瘤细胞对恶性及良性肿瘤的鉴别诊断。方法 :采用吖啶橙荧光法 (AOF)、免疫荧光法(IGF)、血卟啉荧光法 (HOF)检测胸水肿瘤细胞。结果 :11例乳腺癌中三种方法的阳性率均为 10 0 % ;42例肺癌阳性率分别为95 .2 %、95 .2 %、92 .8% ;6 8例肺结核阳性检出率分别为 4.41%、2 .94%、1.47% ;18例肺部感染阳性检出率分别为 1.11%、0 .5 5 %、0 ;5例自发性液气胸和 5例慢性肺源性心脏病均为阴性。结论 :三种荧光法均可用于胸水肿瘤细胞的鉴别诊断。

Objective:[WT5BZ]Discuss the role of fluorescence methods in differentiate diagnosis of carcinoid and malignant tumor cells in thoracic liquid.[WT5HZ]Methods:[WT5BZ]Tumor cells in thoracic liquid of cancer patients diagnosed by pathology were detected by immunoglobulin G fluorescence(IGF),hematoporphyrin fluorescence(HOF)and acridine orange fluorescence(AOF)methods.[WT5HZ]Results:[WT5BZ]In 11 thoracic ciquid samples of breast carcinoma,the positive rates of these three methods were all 100%;The postitve rates of AOF,IGF,HOF are 95.2%,95.2%,and92.8%respectively in 42 lung carcinoma samples;In 68 thoracic liquid samples of pulmonary tuberculosis,the positive rates were 4.41%、2.94%、1.47%;and in about 18 pneumonia samples,they were 1.11%,0.55%、0 respectively;All they were negative in 5 spontaneous fluid pneumothorax and 5 chronic pulmony cardiac disease samples.[WT5HZ]Conclusion:[WT5BZ]The three fluorescence methods have high specificity for tumour cells. [WT5HZ]