表面等离子体共振生物芯片快速检测克伦特罗

A Rapid Method of Biological Chip for Detecting Clenbuterol Based on Surface Plasmon Resonance

盐酸克伦特罗是一种瘦肉精,在中国被禁止使用。根据免疫反应的规律,研究基于表面等离子体共振技术的生物芯片无标记检测盐酸克伦特罗的方法,提出了连续检测法和快速检测法。连续检测法是在检测时交替通入待测样品和PBS缓冲液,检测完成后才通入SDS-HCl,实现芯片再生。该方法可以提高芯片的检测次数,延长使用寿命,适用于检测克伦特罗抗体、筛选抗体、研究免疫反应动力学、建立标准曲线。快速检测法是在生物芯片制备和检测免疫反应过程中动态调整扫描角度,能有效提高检测灵敏度,同时去除冗余的数据,检出限为2 mg/L。检测了猪肉中提取的克伦特罗样品,浓度2.75μg/L。本文所采用的方法具有灵敏度高、操作简便、快速、不需要标记、成本低、设备简单、对环境无污染等优点,有望实现大量样品的现场实时检测。

Clenbuterol Hydrochloride, categorized as brown meat essence, is prohibited in China. Clenbuterol is detected by two methods of continuous and rapid according to the law of immune response. The methods are based on surface plasmon resonance by using the biological chip. They are capable of detecting anti-Clenbuterol without labeling. The continuous method renews the chip by injecting the sample and the PBS buffer solution alternatively and injecting SDS-HCl after detection. The method can increase the detection times and extend the life cycle of the biological chip, which is suitable for detecting anti-Clenbuterol, screening anti-Clenbuterol, studying immunoreaction kinetics and establishing standard curves. In addition, the rapid method can adjust the scan angle dynamically in the process of biological chip preparation and immune response detection, which can effectively improve the sensitivity of the detection, remove the redundant data simultaneously, and achieve the detection limit as high as 2 mg/L. Clenbuterol sample （2.75 μg/L） extracted from pork was detected. The proposed methods showed high sensitivity, user-friendly control, rapid detection, label-free analysis, low cost, simple construction, and environmentally friendly equipment. Both methods could achieve the real-time detection of substantial samples.