应用共振散射光谱法研究香柠檬烯的潜在毒性

Application of Resonance Light Scattering Spectrometry in Assessment of the Potential Toxicity of Bergapten

本文引用DNA嵌入剂理论,通过共振光散射光谱研究了香柠檬烯这种食品调味料分子在大肠杆菌细胞内和体外与DNA分子的相互作用,推算出香柠檬烯分子与DNA分子的结合饱和值,并与溴化乙锭、阿霉素、米托蒽醌、异补骨脂素的结合饱和值进行比较。结果表明:香柠檬烯分子可部分进入大肠杆菌细胞内,其进入细胞和与DNA结合能力比溴化乙锭的弱;在中性环境中,香柠檬烯与DNA的结合饱和值为0.18,溴化乙锭、阿霉素、米托蒽醌、异补骨脂素与DNA的结合饱和值分别为14.70、10.58、3.31、0.34,由此可以推测出:香柠檬烯的毒性远小于溴化乙锭、阿霉素、米托蒽醌的毒性,略小于异补骨脂素的毒性。这为人们快速检测香柠檬烯等类似结构的食品调味剂分子的潜在毒性提供了技术支持和参考。

DNA intercalation theory was applied in study of the interaction between bergapten which was one kind of flavourings and DNA both in E. coli cells and in vitro by resonance light scattering spectroscopy. The DNA saturation binding value with bergapten was calculated and compared with the saturation values of ethidium bromide, adriamycin, mitoxantrone, and isopsoralen. The results showed that bergapten could partly enter E. coli cells and its abilities to both enter the cells and combine with DNA were lower than those of ethidium bromide. In a neutral environment, the DNA-binding saturation value of bergapten was 0.18, while the saturation values with ethidium bromide, adriamycin, mitoxantrone and isopsoralen were 14.70, 10.58, 3.31, and 0.34, respectively. As a consequence, it appeared that the potential toxicity of bergapten was much lower than those of ethidium bromide, adriamycin, mitoxantrone and a little lower than that of isopsoralen. This research provided technical assistance and reference for quickly testing the potential toxicity of bergapten and flavourings with the similar structure of bergapten.