结核分支杆菌耐异烟肼基因inhA突变的测序研究

STUDY ON SEQUENCE ANALYSIS OF GENE inhA MUTATIONS ISOLATED FROM MYCOBACTERIUM TUBERCULOSIS WITH RESISTANCE TO MULTI-DRUGS

目的 :对多耐药株结核分支杆菌的inhA基因进行测序分析。方法 :inhA片段为引物 ,聚合酶链反应扩增产物 ,克隆后制备质粒 ,直接测定DNA序列。结果 :在17个耐异烟肼菌株中 ,11株出现inhA基因变异 ,突变率为64.7%。主要为碱基置换和缺失 ,各菌株变异不同。katG和inhA同时出现变异的比例高。结论 :结核菌的耐异烟肼 (INH)和乙胺丁醇 (EMB)变异与inhA突变有关 。

Objective: To determine the sequence of gene inhA mutations isolated form M tuberculosis with resistance to multi-drugs. Method: the primer was from inhA patch and the products were produced by PCR and purified cloned and analyzed by ABI 377 auto-DNA sequencing system. Results: 11 out of isolated 17 strains with resistance to multi-drugs were detected to have inhA variations (mutation rate 64.7%) , which mainly showed substitutions and deletion. The variations were different from strain to strain. Conclusion: both INH-resistant and EMB- resistant variations of M tuberculosis have the relationship to inhA mutations, mainly point mutations.