口腔粘膜下纤维性变组织中内皮素1的免疫组织化学和定量研究

Quantitative and Immunohistochemical Analysis of Endothelin-1 in Oral Submucous Fibrosis

目的 :探讨ET 1在口腔粘膜下纤维性变 (OSF)发病机制中的作用。方法 :采用免疫组化染色SABC法和图像分析技术 ,对OSF早、中、晚期各 10例、口腔扁平苔藓 (OLP) 10例以及正常人 10例的颊粘膜组织中ET 1表达进行定量分析。结果 :①OSF组织中ET 1免疫阳性物质超量表达 ,正常及OLP组织中ET 1表达微弱 ;②OSF早、中、晚期组织中ET 1含量显著高于正常 (P <0 0 1) ,且早、中期显著高于晚期 (P <0 0 1) ;③OSF早、中期组织ET 1含量显著高于OLP(P <0 0 1) ,晚期间质ET 1含量显著高于OLP(P <0 0 5 ) ,上皮ET 1含量两者差异不显著 (P >0 0 5 ) ;④口腔粘膜中上皮和间质的ET 1含量呈显著正相关 (P <0 0 5 )。结论 :ET 1在OSF的表达具有特异性 ,且可能影响OSF病变的发生发展。

Objective:This experiment is carried out to investigate roles of endothelin 1(ET 1),a potent vasoconstrictor and mitogenic peptide,in fibrosis and collagen production. Methods: 30 cases of oral submucous fibrosis(OSF) ,10 cases of oral lichen planus(OLP) and 10 cases of normal control, were studied by means of SABC immunohistochemical method with polyclonal antisera to ET 1 and using image pattern analysis technique. Results: ①Oral mucosa from OLP and normal control expressed very little ET 1 .By contrast,there was striking expression of ET 1 in oral mucosa from OSF;②The cellular content of ET 1 in OSF was significantly higher than that in normal control (P<0.01),while the cellular content of ET 1 in the early and moderate stages OSF were significantly higher than that in the advanced stage(P<0 01);③The cellular content of ET 1 in the early and moderate stages OSF were significantly higher than that in OLP(P<0 01);④There was a significantly positive correlation between cellular contents of epithelia and interstitia(P<0 05). Conclusion:These findings suggest that cell specific expression of ET 1 may play a role in pathogenesis of OSF.\;