CGRP对大鼠缺血再灌注后海马内突触体素表达的影响

Effect of CGRP on Synaptophysin in the Hippocampus of the Ischemic and Reperfusion Rat

为了探讨降钙素基因相关肽 CGRP对大鼠脑缺血再灌注后突触体素 (synaptophysin,p38)表达的影响 ,我们采用暂时夹闭双侧颈总动脉法制作大鼠脑缺血再灌注模型 ,经右侧颈内动脉主入 CGRP,采用单克隆抗 p38抗体免疫组织化学染色及显微图像方法研究 CGRP对大鼠再灌注海马内 p38的影响。结果表明 :大鼠缺血再灌海马内 p38反应产物较正常组显著降低 (P <0 .0 1) ,而注射 CGRP组阳性产物明显高于缺血再灌注组 (P <0 .0 1)。这说明大鼠缺血再灌注 p38表达明显减少 ,而 CGRP可上调缺血再灌注 p38的表达。本文结果提示 :CGRP参与缺血神经元突触体素的调节 ,CGRP对缺血神经元有一定的修复作用。

To study the effect of CGRP on synaptophysin in the hippocampus of the ischemic and reperfusion rat, we established the model of ischemic reperfusion rat by tentatively clamping bilateral common carotid arteries. Immunohistochemical technique and microimage analysis were used to estimate the effect of CGRP on the expression of synaptophysin in ischemic neurons in hippocampus. The results showed that the expression of p38 was signifcantly decreased after cerebral ischemic and reperfusion, and CGRP increased the expression of p38. These results indicate that CGRP participates in the regulation of synaptophysin in ischemic neurons, has some recovery effect on ischemic neurons.