凋亡细胞促进狼疮性BXSB小鼠发病的研究

Apoptotic cells accelerate the development of systemic lupus erythematosus in lupus prone BXSB mice

目的 探讨凋亡细胞在系统性红斑狼疮发病中的作用。方法 将体外诱导的凋亡胸腺细胞经腹腔注射给同基因同性别的正常C5 7小鼠和具有狼疮倾向的雌雄BXSB小鼠 ;检测血清中IgM和IgG类型的抗dsDNA抗体和抗ssDNA抗体的水平 ,尿蛋白浓度以及肾脏中IgG类型免疫复合物的沉积。结果 用凋亡胸腺细胞免疫后 ,诱导C5 7小鼠产生IgG类型的抗dsDNA抗体和抗ssDNA抗体 ;相比之下 ,诱导雌性BXSB小鼠产生更高水平的IgG类型抗dsDNA抗体和抗ssDNA抗体 ;并且使雄性BXSB小鼠的尿蛋白浓度显著升高。结论 凋亡细胞具有免疫原性 ,它可能是狼疮性BXSB小鼠体内自身抗原的主要来源 ;

Objective To probe into the role of apoptotic cells in the disease development of systemic lupus erythematosus.Methods Apoptotic thymocytes injected intraperitoneally into syngeneic normal C57 mice and lupus prone BXSB mice,and then the level of anti dsDNA and anti ssDNA antibodies of IgM and IgG classes,and the concentration of urine protein and immune complex of IgG class deposited in kidney were detected.Results The apoptotic thymocytes induced the production of anti dsDNA and anti ssDNA antibody of IgG class in C57 mice,and their levels were higher in female BXSB mice than in male ones.The concentration of urine protein of male BXSB mice injected with apoptotic thymocytes was noticeably higher than that of the control male BXSB mice.Conclusion Apoptotic cells have immunogenicity,and may be the major source of autoantigens in lupus BXSB mice.Lupus genetic factors and Yaa gene can potentiate the immunogenicity of apoptotic cells or accelerate their inducing the lupus nephritis.