AG490对大鼠脑损伤后CD40和CD45表达及神经功能的影响

Effects of AG490 on the expression of CD40 and CD45 and neurological functional status after brain injury in rats

目的探讨AG490对大鼠创伤性脑损伤后脑组织CD40、CD45表达以及神经功能状态的影响。方法取健康雄性SD大鼠180只,随机分为假手术组、创伤组和AG490干预组,每组大鼠60只;各组根据脑损伤时间再分为6、12、24及72h亚组(各15只)。应用液压冲击法制备大鼠脑损伤模型,采用流式细胞术检测脑组织CD40、CD45的表达,采用大鼠神经功能缺损评分系统对神经功能状态进行评估。结果①创伤组伤后各时间点神经功能缺损评分均高于假手术组(P<0.05或P<0.01),其中6 h为高峰期,后呈现逐渐下降趋势。AG490干预组大鼠神经功能缺损评分均较创伤组低,24和72 h时间点差异有统计学意义(P<0.05或P<0.01)。②创伤组脑组织CD40表达水平逐渐升高,24 h达高峰,然后开始降低;CD45表达持续增高,72 h最高。与假手术组比较,各时间点CD40、CD45表达差异均有统计学意义(P<0.01)。AG490干预组各时间点CD40、CD45表达水平均低于创伤组,差异均有统计学意义(P<0.01)。结论创伤性脑损伤后,AG490可能通过降低脑组织CD40、CD45的表达促进神经功能的恢复。

Objective To investigate the effects of AG490 on the expression of CD40 and CIM5 in cerebral tissue and neurological functional status after traumatic brain injury in rats. Methods A total of 180 healthy male SD rats were randomly allocated into a sham operation, a traumatic brain injury, and an AG490 intervention group （n =60 in each group）. The rats in each group were redivided into 6, 12, 24 and 72 h subgroups according to the time of brain injury. A brain injury model was induced by using a hy- draulic shock method. The CD40 and CD45 expression in brain tissue was detected by flow cytometry. The rat neurological deficit score was used to judge the neurological status. Results （~The neurological defi- cit scores of all time points in the traumatic brain injury group were higher than those in the sham operation group （P 〈0.05 or P 〈0.01 ）, it reach the peak at 6 h, and then gradually downward. The neurological deficit scores in the AG490 intervention group were lower than those in the traumatic brain injury group. There was significant difference between the time points of 24 h and 72 h （P 〈 0.05 or P 〈 0.01 ）. （~）The CD40 expression level in brain tissue in the traumatic brain injury group increased gradually, it reached the peak at 24 h, and then it began to decrease ; the CIM5 expression level increase continuously, it reached the peak at 72 h. Compared with the sham operation group, there were significant differences at each time point between the CD40 and CD45 expression （P 〈0.01 ） ; the CD40 and CD45 expression levels at eachtime point in the AG490 intervention group were lower than those in the traumatic brain injury group. There were significant differences （P 〈 0.01 ）. Conclusion After traumatic brain injury, AG490 may promote the recovery of neurological function by reducing CD40 and CD45 expression in brain tissue.