Wnt5a在严重烫伤大鼠早期肠黏膜组织中的表达及意义

Expression of Wnt5ain Intestinal Mucosa of Rats During the Early Stage After Severe Burn Injury

目的:研究在严重烫伤大鼠肠黏膜组织中Wnt5a动态表达变化及其意义。方法:选取48只SD大鼠,将大鼠背部浸入恒温水浴盆(92℃)中20s达Ⅲ度烫伤,制作烫伤创面,采用随机数字法将大鼠分为对照组、单纯烫伤组(烫伤组)、烫伤后胰岛素治疗组(治疗组)。伤后取肠组织观察大体变化,取肠道黏膜HE染色后,行组织形态学观察,分别于烫伤后6,12,24,48h四个时相点观察,取肠黏膜组织以免疫组织化学的方法,检测Wnt5a的表达情况。结果:在烫伤组和治疗组Wnt5a均有显著表达,对照组有少量表达(P<0.05),伤后6-12hWnt5a的表达持续升高,烫伤组于12h开始表达逐渐减少,24h后表达又重新增多,治疗组在48h表达达到最高峰,与烫伤组相比,治疗组Wnt5a的表达水平明显降低(P<0.05),病理形态学变化显示烫伤早期大鼠肠黏膜组织损伤明显,治疗组大鼠肠黏膜组织损伤较烫伤组明显减轻。结论:严重烫伤早期SD大鼠肠黏膜组织损伤明显,Wnt5a可能是反映烧伤脓毒症的重要标志。

Objective： To investigate the changes of expression of WntSa in intestinal mucosa of rats dur- ing the early stage after severe burn injury. Methods： Scald models were set up in 48 healthy SD rats, which were randomly divided into control group, scald group and scald q- insulin group （treatment group）. The intestinal mucosa tissue samples were extracted separately at 6th, 12th, 24~th, and 48th hours after scald injury. The pathological changes of the intestinal mucosa tissue of each group were observed, and the expression of Wnt5a in the intestinal mucosa was detected with immunohistochemical methods and analyzed by a micro-image analysis system. Results： In the scald group, the expression of WntSa was higher than that in control group at the same time （P^0.05）. In the first 12 hours post-scald injury, the WntSa expression in control group was in- creasing, and then decreased obviously from the 12th hour, and after 24 hours, it was return to increase. In treatment group, the expression reached the peak at the 48th hour post-scald injury.The pathological changes were milder in treatment points. Conclusion： In the early phase of severe burn very severe. WntSa may take part in the procession protecting the intestinal mucosa tissue. group than in control group in each time injury, the intestinal mucosa tissue injury is of burn sepsis which may be one of factors