麻黄附子细辛汤活性部位对RAW 264.7细胞活力的影响

The effect of the active parts and components of Mahuangfuzixixin Decoction on RAW 264.7 cells

目的:探讨麻黄附子细辛汤活性部位及成分对LPS诱导的巨噬细胞RAW 264.7活力的影响。方法:用1μg/ml LPS诱导RAW264.7细胞使之增殖,同时,麻黄附子细辛汤活性部位及成分以对细胞无毒性的的浓度进行实验,以MTT法检测细胞的活力变化。结果:麻黄总碱(100μg/L)、麻黄碱、甲基麻黄碱、伪麻黄碱、苯甲酰次乌头原碱、苯甲酰乌头原碱在10μg/L^100μg/L浓度范围内和细辛挥发油(2.2μl/L)均有降低LPS诱导的巨噬细胞RAW 264.7增殖的作用,与对照组(LPS组)相比差异具有统计学意义,而附子总碱及苯甲酰新乌头原碱、乌头碱、次乌头碱、新乌头碱、去甲基麻黄碱、去甲基伪麻黄碱无此作用。结论:麻黄附子细辛汤中不同活性部位及成分,对RAW 264.7细胞活力的影响不同。

To research the effect of the active parts and components of Mahuang-Fuzi-Xixin Decoction on RAW 264.7 ceils which was induced by lipopolysaccharide. Methods： As the cells was deduced by lipopolysaccharide （1μg/ml）, the non-toxic concentrations of the ac tive parts and components of Mahuang-Fuzi-Xixin Decoction were selected to be done, the change of viability of the cells was observed by MTY method. Results： The total alkaloids of Mahuang （ 100μg/L）, volatile oil of Xixin （2.2μl/L） and ephedrine, methylephedrine, pseudoephedrine, Benzoylhypacoitine, benzoylaconine at the concentration of 10μg/L to 100μg/L could inhibited the proliferation of RAW 264.7 induced by lipopolysaccharide, compared with controlled group, the differences was siginificant （ P 〈 0.05 ）. The others had no this effect. Conclusion： The effects were different of the active parts and components of Mahuang-Fuzi-Xixin Decoction on RAW 264.7 cells.