没药烷吉酮治疗痛经及机制研究

Studys on anti-dysmenorrhea effects and mechanism of bisabolangelone

目的:研究没药烷吉酮对痛经的影响,并初步探讨其作用机制。方法:观察没药烷吉酮灌胃给药后对小鼠痛经模型的镇痛作用;采用在体、离体子宫实验法,观察没药烷吉酮对缩宫素所致子宫收缩的对抗作用;并利用离体子宫实验考察没药烷吉酮对CaCl2、KCl、乙酰胆碱所致子宫收缩的影响,初步探讨其治疗痛经的作用机制。结果:没药烷吉酮在4 mg/kg、8 mg/kg下对小鼠痛经模型具有明显镇痛作用;没药烷吉酮在有效浓度(1.2μg/ml、1.6μg/ml)下对缩宫素所致在体、离体子宫收缩均具有明显对抗作用;没药烷吉酮(1.6μg/ml)能同时明显抑制CaCl2、KCl、乙酰胆碱所致离体子宫的收缩。结论:没药烷吉酮具有治疗痛经的作用,其作用机制与钙拮抗有关。

To study the effect and the mechanism of bisabolangelone on treatment of dysmenorrhea. Method： The analgesic effect of bisabolangelone on dysmenorrhea model was observed in mice after oral administration. The experiments method of uterus in vivo and in vitro was adopted, and antagonism of bisabolangelone on uterine contractions caused by oxytocin was tested. Using the isolated uterus experiment, we invesitegated the effect of bisabolangelone on uterine contraction induced by CaC12, KCI, acetylcholine, and explored its mechanism in treating dysmenorrhea. Result： Bisabolangelane had a significant analgesic effect on mice dysmenorrhea model under the experimental doses （4mg/ kg,8 mg/kg）. Bisabolangelone had the inhibitory effect on uterine contractions induced by oxytocin in vivo and in vitro under the effective concentration （1.2 μg/ml, 1.6 μg/ml）. The concentration of bisabolangelone（ 1.6 μg/ml） also inhibited uterine contractions induced by CaC12, KCI, acetylcholine in vitro simultaneously. Condutlon： Bisabolangelone had a good effect in treating dysmenorrhea, its mechanism of action was related with calcium antagonism.