黄芪丹参配伍对局灶性脑缺血再灌注大鼠脑组织VEGF和bFGF mRNA表达的影响

Effects of combination of Astragalus and Salvia on the expression of VEGF and bFGF mRNA against focal cerebral ischemia-reperfusion injury in rats

目的:观察黄芪注射液配伍丹参注射液对脑缺血/再灌注损伤大鼠的保护作用并分析可能的作用机制。方法:雄性SD大鼠随机分为假手术组、模型组、黄芪注射液组(6 g/kg)、丹参注射液组(6 g/kg)、黄芪注射液配伍丹参注射液组(黄芪3 g/kg,丹参3 g/kg)和尼莫地平对照组(1 mg/kg)共6组。制备大脑中动脉阻塞模型,脑缺血2 h/再灌22 h进行神经功能评分,应用TTC染色测量脑梗死范围,Real time PCR技术检测大鼠脑组织血管内皮细胞生长因子(VEGF)、碱性成纤维细胞生长因子(bFGF)mRNA的表达。结果:与模型组比较,黄芪注射液配伍丹参注射液能明显降低模型大鼠的神经功能评分,减小脑梗死范围,同时能上调脑组织VEGF、bFGF的mRNA表达,作用优于单用黄芪注射液组或单用丹参注射液组。结论:益气活血法(黄芪注射液配伍丹参注射液)比单用益气法(黄芪注射液)或单用活血法(丹参注射液)能更有效地降低脑缺血/再灌注损伤大鼠大鼠神经功能评分,减小脑梗死范围,同时上调脑组织VEGF、bFGF的mRNA表达从而达到脑保护作用,这可能是临床应用益气活血法治疗缺血性中风的机理之一。

To investigate the protection of supplementing qi and activating blood circulation therapy （ combination of Astragalus and Sal via injection） on focal crerebral ischemic-reperfusion injury in rats and its possible mechanisms. Methods： Male SD rats were randomly divid ed into sham-operation group, model group, Astragalus injection group（6 g/kg）, Salvia injection group （6 g/kg）, combination of Astragalus and Salvia injection group（3 g/kg and 3 g/kg）, and Nimodipine injection group（ 1 mg/kg）. The method of middle cerebral artery occlusion in rats （middle cerebral artery occlusion, MCAO） was adopted to establish cerebral iscbemia model, after cerebral ischemia 2 h/reperfusion 22 h, evaluation index like cerebral infarction volume and behavioral symptoms of brain were taken account in terms of combination of Astrag alus and Salvia injection in cerebral ischemia neural protection. Real time PCR was applied to detect vascular endothelial growth factor （VEGF） and basic fibroblast growth factor （bFGF） mRNA expression on ischemic cerebral cortex. Results： Compared with model group, combination of Astragalus and Salvia injection could significantly improve brain behavior signs （ P 〈 0.05 ） and reduce the infarction volume （P 〈0.01 ）, and could up-regulated VEGF and bFGF mRNA expression in brain tissue of model rat （ P 〈0.01 ）, better than simply using Astragalus or Salvia injection group. Conclusion： Supplementing qi and activating blood circulation therapy （combination of Astragalus and Salvia injection） was better than single supplementing qi （Astragalus injection） and single activating blood circulation （Salvia injection）, which can more effective reduce cerebral ischemia/reperfusion injury in rat on nerve function score, reduce infarct volume, while up-regulated VEGF and bFGF mRNA expression in brain tissue. This may be one of the mechanisms of supplementing qi and activating blood circulation therapy used for ischemic stroke in clinical applications.