摘要
协同应用纤维素酶和木质素酶催化降解废次烟叶,探讨清洁高效的酶解破壁效应及浸提茄尼醇工艺条件。结果发现复配酶催化裂解溶浸茄尼醇效果明显优于单一酶,酶解时间、温度、pH值以及酶投加量等条件均影响酶破壁浸提茄尼醇能效。结果表明,采用纤维素酶:木质素酶酶活比15∶1(U/U)的复配酶,在体积为5倍烟草质量的水介质环境中,当复配酶投加量为175 U/g,水浴温度40℃,pH为6时,催化酶解烟叶8 h后,茄尼醇溶浸浓度可达0.33 g/L。在此条件下,茄尼醇平均提取率可达96.53%,是化学回流浸提方法的1.68倍。该方法为有效提取废次烟草中茄尼醇提供了一种新途径。
To improve the extraction of solanesol from tobacco waste, we developed an enzymatic cell wall-breaking process with combined cellulase and ligninase. The effects of reaction time, temperature, pH and enzyme/substrate ratio were determined. The results show that the catalytic effect was better than either single enzyme when the ratio of cellulase to ligninase was 15:1 (U/U). Under the optimized conditions of 175 U/g (enzymes/substrate), tobacco to water 1:5 (W/W), temperature 40 ℃ and pH 6.0, the concentration of solanesol in the solution could reach 0.33 g/L after 8 h. And the average leaching rate reached 96.53% which was 1.68 times of the extraction methods of chemical reflux. It provides new way for the extraction of solanesol from tobacco waste, and worthwhile to be further explored.
出处
《生物工程学报》
CAS
CSCD
北大核心
2013年第11期1706-1710,共5页
Chinese Journal of Biotechnology
基金
重庆市科技攻关项目(No.cstc2011AB7060)
重庆市教委资源化学与利用项目(No.KJTD201020)资助~~
关键词
酶促反应
茄尼醇
废次烟草
破壁
提取
enzymatic reaction, solanesol, tobacco waste, wall-broken, extraction