酶解破壁促进废次烟叶中茄尼醇溶浸

Improved extraction of solanesol from tobacco waste by enzymatic cell wall breaking

协同应用纤维素酶和木质素酶催化降解废次烟叶,探讨清洁高效的酶解破壁效应及浸提茄尼醇工艺条件。结果发现复配酶催化裂解溶浸茄尼醇效果明显优于单一酶,酶解时间、温度、pH值以及酶投加量等条件均影响酶破壁浸提茄尼醇能效。结果表明,采用纤维素酶:木质素酶酶活比15∶1(U/U)的复配酶,在体积为5倍烟草质量的水介质环境中,当复配酶投加量为175 U/g,水浴温度40℃,pH为6时,催化酶解烟叶8 h后,茄尼醇溶浸浓度可达0.33 g/L。在此条件下,茄尼醇平均提取率可达96.53%,是化学回流浸提方法的1.68倍。该方法为有效提取废次烟草中茄尼醇提供了一种新途径。

To improve the extraction of solanesol from tobacco waste, we developed an enzymatic cell wall-breaking process with combined cellulase and ligninase. The effects of reaction time, temperature, pH and enzyme/substrate ratio were determined. The results show that the catalytic effect was better than either single enzyme when the ratio of cellulase to ligninase was 15：1 （U/U）. Under the optimized conditions of 175 U/g （enzymes/substrate）, tobacco to water 1：5 （W/W）, temperature 40 ℃ and pH 6.0, the concentration of solanesol in the solution could reach 0.33 g/L after 8 h. And the average leaching rate reached 96.53% which was 1.68 times of the extraction methods of chemical reflux. It provides new way for the extraction of solanesol from tobacco waste, and worthwhile to be further explored.