佛手种质资源遗传多样性的ISSR分析

Genetic Diversity Analysis of Fingered Citron Germplasm by ISSR

收集佛手（CitrusmeicaL．vat．sarcodactylisSwingle）13份、枸橙（cmedicavaEyunnanensisDing）1份及香椽（Cme~caL．）6份，共20份种质资源，其中14份成功引种在佛手资源圃。在对其中14份资源生物学特性进行描述的基础上，采用ISSR分子标记技术对20份样品进行遗传多样性分析。筛选出的11条引物共检测到102条DNA条带，其中58条为多态性条带，多态性条带比率为56．9％。各样品之间的遗传相似性系数在0．608—0．990之间。聚类分析结果表明，当遗传系数为O．798时，可将供试材料分为枸橙、香橼和佛手3个类群，新发现的3个芽变材料是佛手中一个新的类群。

Thirteen fingered citron accessions, 1 citrate acid orange and 6 citron accessions were collected and fourteen genotypes were introduced and survived in the germplasm resources garden. Based on the biological traits, the genetic diversities of 20 samples were evaluated by ISSR markers. A total of 102 DNA bands were amplified from the 11 polymorphic primers, which were screened from 100 ISSR primers. Among those obtained bands, 58 were polymorphic ones, accounting for 56.9% polymorphism. The genetic similarity coefficient among the accessions ranged from 0.608 to 0.990. At the point of the similarity coefficient 0.798, all the accessions might be separated into three groups, fingered citron, citrate acid orange and citron, meanwhile three pieces of Yabian mutants belonged to a new group of fingered citron.