高效液相色谱法测定活血复脉合剂中原儿茶醛的含量

Determination of protocatechuic aldehyde in Huoxue Fumai mixture by HPLC

目的建立高效液相色谱法测定活血复脉合剂中原儿茶醛的含量。方法采用反相高效液相色谱法,流动相为甲醇-0.5%冰醋酸溶液(20:80,V/V),检测波长为280 nm,流速为1.0 mL·min^(-1),进样量为20μL,柱温为25℃。采用峰面积外标法定量。结果原儿茶醛进样浓度在5.437 5~174μg·mL^(-1)范围内与峰面积积分值呈良好的线性关系(r^2=1,n=5),平均加样回收率为99.4%,精密度为0.51%。结论该法专属性强,简便准确,精密度、稳定性、重复性良好,可以用于活血复脉合剂的质量控制。

AIM To establish a method for the determination of protocatechuic aldehyde in Huoxue Fumai mixture, providing evidence for its quality standard. METHODS RP-HPLC was adopted with mobile phase consisted of methanol-0.5% acetic acid solution （20 ： 80, V/V） at the flow rate of 1.0 mL·min-1. The detection wavelength was set at 280 nm and column temperature was 25℃. The injection volume was 20 μL. RESULTS The linear range of protocatechuic aldehyde was 5.437 5 - 174 μg·mL-1 （r2 = 1, n = 5） with average recovery rate of 99.4% （RSD = 0.51%）. CONCLUSION The method is specific, simple and accurate with a good precision, stability and reproducibility, which can be used for the quality control of Huoxue Fumai mixture.