微量发色三肽底物法测定抗凝血酶Ⅲ活性及其临床应用

Determination of antithrombinⅢ activity by microassay of chromogenic tripeptide substrate and its clinical application．

目的与方法 用微量发色底物法测定ATⅢ活性并初步应用于临床.结果ATⅢ活性在25%～150%范围内与吸光度成线性关系.批内CV＝4．8%,批间CV＝5．8%.66例正常人血浆ATⅢ活性为101．1±31．8%(x±2s),65例各型肝炎(除急性肝炎外)和23例肾病综合症及18例尿毒症病人血浆ATⅢ活性明显降低.结论 该方法底物用量少,成本降低,重复性好,结果可靠,简便实用,可用于常规检验.

Objective and Method Determination of antithrombin Ⅲ ATⅢ activity by microassay of chromogenic tripetide substrate and tentative clinical application． Results A linear relationship was obtained between ATⅢ activity and optical density in the 25%～150% activity range． The Within－run was CV＝4．8% and between－run was 5．8%． The ATⅢ activity range of plasma of 66 normal subjects was 101．1±31．8%x±2s while that of 65 patients with liver diseases except acute hepatitis and 23 patients with nephrotic syndrome and 18 patients with uremia decreased significantly． Conclusion This method demands only small amount of substrate so that the cost can be redeuced． It is simple repeatable reliable practical and suitable for routine clinical test． 