金丝桃苷对CCl_4诱导大鼠急性肝损伤抗氧化应激研究

Effect of hyperin on acute liver injury in rats against oxidative stress-induced by CCl_4

目的研究金丝桃苷(Hyp)对四氯化碳(CCl4)诱导大鼠急性肝损伤的治疗作用。方法采用大鼠CCl4急性肝损伤模型,观察Hyp对急性肝损伤大鼠肝脏组织病理学改变的影响;检测肝组织匀浆中超氧化物歧化酶(T-SOD)、谷胱甘肽(GSH)的活性及丙二醛(MDA)含量变化。结果 CCl4模型组大鼠肝组织HE染色病理检测结果见明显炎症变性坏死及纤维组织增生现象;Hyp高剂量60 mg/kg、中剂量30 mg/kg治疗组的肝组织病理改变明显改善;Hyp治疗组肝组织中T-SOD、GSH活性明显升高,MDA含量明显降低,并存在量效关系。结论 Hyp对CCl4引起的大鼠急性肝损伤有较好的治疗作用,其机制可能与其抗氧化活性有关。

Objective To study the protective effect of hyperin（Hyp） on the acute liver injury in rats induced by CCl4. Methods The acute liver injury model was induced by CCl4. The effect of Hyp on acute liver injury of rat liver histopathology was observed ; and the impact of changes of homogenates total superoxide dismutase （T-SOD）, glutathione （GSH） activity and malondialdehyde （MDA） in liver were de-tected. Results The acute liver injury of tissue induced by CCl4, apparent pathological inflammation and fibrous tissue degeneration and necrosis were observed by HE staining ; At the high doses of 60 mg/kg and medium doses of 30 mg/kg treated by Hyp,liver pathology changes was significantly obvious. The T-SOD, GSH activity of liver tissue was significantly increased in the groups of Hyp treatment, MDA content was significantly decreased, which related to the dosages. Conclusion In the rat model of acute liver injury induced by CCl4. A better therapeu-tic effect of Hyp was shown, the mechanism may be related to its antioxidant activity.