摘要
目的探讨苯丙胺对黑质DA神经元损伤的机制。方法将60只大鼠随机分为3组:空白组10只、生理盐水组10只、苯丙胺处理组40只。苯丙胺处理组又随机分为苯丙胺给药(剂量为2.5 mg·kg-1·d-1)后1 h、1 d、7 d和14 d组,每组各10只。观察给药后的行为学变化,再用Western blot方法检测各组黑质LaminB1和GRP94蛋白的表达量。结果苯丙胺在首次给药后就可引起大鼠自主活动量的增加,并且随着给药时间的延长出现了刻板行为;Western blot方法检测黑质GRP94和LaminB1蛋白的表达在各组间未见差异。结论低剂量短时间苯丙胺对黑质DA能神经元的损害可能与GRP94和LaminB1蛋白的变化没有关系。
Obiective To explore the mechanism of amphetamine-induced injury in substantia nigra dopamine neurons. Methods SD rats were randomly divided into control, saline and amphetamine groups（which were subdivided into 1 h, 1 d,7 d, 14 d groups by medication time）. Observing the changes of rats＇ behavior after the administration and using western blot to detect the expression of GRP94 and LaminB1. Results After the first time to intraperitoneal injecting amphetamine, the increase of self- activity in rats can be found. And as time go on, there had been stereotyped behaviors. In the substantia nigra among the groups, there wasn＇ t any significant difference of the expression of GRP94 and LaminB1 can be detected in Western blot. Conclusion It＇ s possible that there were no significant associations between amphetamine-induced injury of dopamine neurons with expression of GRP94 and LaminB1 in substantia nigra on low-dose and short time.
出处
《解剖学研究》
CAS
2013年第5期326-330,共5页
Anatomy Research
