不同品系小鼠对mCPP诱导焦虑样行为的差异研究

Differences in anxiety-related behavior induced by mCPP and responses to diazepam in two strains of mice

目的:评价BALB/c和ICR小鼠对1-(3-氯苯基)哌嗪单盐酸盐(mCPP)诱导焦虑样行为的差异。方法:将两品系小鼠各分为四组:生理盐水对照组、mCPP焦虑组、地西泮对照组和地西泮+mCPP组。行为学检测前30min腹腔注射地西泮、皮下注射mCPP,采用开场实验、明暗穿箱和高架十字迷宫比较两品系小鼠焦虑样行为的差异。结果:地西泮使焦虑的BALB/c小鼠中央路程比值和中央时间比值增加,对ICR小鼠无作用;两品系小鼠运动总路程有差异。地西泮使焦虑小鼠进入暗箱潜伏期延长、明暗穿箱次数增加、暗箱滞留时间缩短,BALB/c小鼠对地西泮的反应比ICR小鼠更敏感。地西泮可使焦虑小鼠开臂进入次数百分比、开臂停留时间百分比和开臂探臂时间百分比增加,两品系小鼠仅在开臂停留时间和开臂探臂时间上有差异。结论:mCPP诱导焦虑小鼠比正常小鼠对地西泮的敏感性高;BALB/c小鼠焦虑水平较低,探究能力和风险评估能力较高,是抗焦虑药物研究中可供选择的较合适的动物品系。

AIM： To compare the differences in anxietyrelated behavior induced by chloro phenylpiperazinehydrochloride （mCPP） and re sponse to diazepam in BALB/c mice and ICR mice. METHODS： Each were divided into four groups： saline group, mCPP group, diazepam group and （diazepam＋mCPP） group. Diazepam was administrated intraperitoneally and mCPP was administrated subcutaeously 30 rain before behavioral test. Openfield test, lightdark tran sition test and elevated plus maze test were used. RESULTS： In openfield test, the central distance ratio and the central time ratio were in creased in the （diazepam Jr mCPP） group of BALB/c mice. But there were no effects on ICR mice. Only was there significant difference on to tal distance between two mice strains. In light dark transition test, latency into dark box and number of transitions were increased, and time ratio in dark box was decreased in （diazepam＋mCPP） group of two strains mice. The response to diazepam was were more sensitive in BALB/c mice than ICR mice. Number of openarm en tries, time and exploring time ratio in openarm in the elevated plus maze all increased in diaze pamqmCPP） group of two strains mice. There were differences of time and exploring time in openarm between them. CONCLUSION： The re sponse to diazepam is more sensitive in anxiety mice than normal mice. BALB/c mice have low level of anxiety, high baseline exploration and risk assessment of a new environment, which is a suitable mice strain to develop anxiolytic drugs.