SRK-SCR转基因拟南芥自交不亲和性的研究进展

Advance in Self-Incompatibility of Arabidopsis thaliana with the Transgetic SRK-SCR

十字花科植物自交不亲和性(SI)受S-位点(S-locus)编码的SRK和SCR控制,它们分别是柱头和花粉中的SI特异识别因子。野生型拟南芥不具有SI,而近来通过转基因手段将外源SRK-SCR基因转入野生型拟南芥可以使其表现SI,由此建立了一个可用于十字花科SI研究的新型模式植物。本文综述了利用这种转基因拟南芥在SI机制及进化方面取得的进展,包括SI新基因的挖掘、候选基因功能分析和拟南芥生殖模式的转变等。

In the Brassicaceae, self-incompatibility （SI） is controlled by the S-locus receptor kinase （SRK） and the S-locus cysteine-rich （SCR） protein. These two proteins are the SI specificity determinants in stigma and pollen, respectively. Recently, the Arabidopsis thaliana with SI phenotype has been achieved by transferring SRK/SCR allelic pairs from self-incompatible relative species to wild type A. thaliana. The new model plant was applied in the mechanism and evolution research of SI in Brassicaceae. This review summarizes the ad- vances in utilizing transgenic A. thaliana SI model, including screening of new SI genes, function analysis of candidate genes and investigation of evolutionary switches to self-compatibility （SC）.