利用κ-卡拉胶-魔芋多糖复配胶固定化酵母生产三磷酸腺苷

Study on Production of ATP by Immobilized Beer Yeast Cells

利用κ 卡拉胶 魔芋多糖复配胶包埋的固定化啤酒酵母细胞 ,由腺苷 (AR)生产三磷酸腺苷 (ATP)。采用HPLC法分析固定化细胞反应过程核苷 (酸 )的变化以及用琼脂糖平板电泳法对其工艺条件进行了优化。固定化细胞反应 1h ,ATP转化率接近 10 0 %。底物溶液灭菌和添加NAD+后 ,该固定化酵母细胞可反复使用 12次 ,ATP的转化率稳定维持在 95 %以上。

The immobilized beer yeast cells entrapped in κ Carrageenan Koniak polysaccharide gel were used to biosynthesize ATP from AR The changes of nucleoside(nucleotides) during reaction course and optimal reaction conditions were researched by HPLC and electrophoresis respectively The conversion rate of ATP could reach almost 100% after 1 hour The immobilized cells could be reused 12 times when substrates were sterilized and NAD + was added