不同年龄C_(57)小鼠海马NGF m RNA含量的测定

Change of βNGF mRNA Levels in Hippocampus from C_(57) Mice of Different Age

采用异硫氰酸胍法从C57小鼠海马中分离总RNA ,以肌动蛋白基因为内标用RT PCR法测定不同年龄小鼠海马NGFmRNA含量的变化。C57小鼠海马NGFmRNA含量在 6月龄和 12月龄明显低于 1月龄 ,而 6月龄与

NGF mRNA levels in hippocampus from C 57 mice of different age were compared by Semi quantitative RT PCR method, taking β actin gene as a control The levels of 6 and 12 month old C 57 mouse were much lower compared to that of 1 month old mouse, however, there was no significant difference between those two groups DNA sequencing has revealed the No 294 base of C 57 mouse β NGF to be C instead of T as usual Nevertheless, this mutation in nucleotide does not make any difference in amino acid composition