江西眼镜蛇毒神经毒素的提纯及部分性质鉴定

Purification and Partial Characterization of A Neurotoxin(CBTX) from Jiangxi Cobra(Naja Naja Atra) Venom

采用CM SephadexG5 0和SP SephadexG5 0两次柱层析 ,从江西眼镜蛇毒中分离提纯得到电泳纯的神经毒素 ,它对大鼠膈神经膈肌标本及清醒家兔具有典型的箭毒样作用 ,小鼠sc的LD50 为 5 3μg/kg ,等电点为 pH8 8,相对分子质量为 6 80 0～ 70 0 0 。

The purification of the most toxic component CBTX from Jiangxi cobra venom was accomplished by column chromatography on CM Sephadex G50 and SP Sephadex G50 column It was found to be homogeneous by polyacrylamide gel electrophoresis CBTX has a typical curamimetic action in conscious rabbits and on the rat phrenic diaphram preparation Its LD 50 to mice(s c ) was 53 μg/kg The isoelectric point of CBTX was pH8 8 Its molecular weight was 6 800 to 7 000 The composition of amino acid of CBTX was similar to Cobrotoxin from Taiwan cobra venom