HPLC测定芪参颗粒中丹参酮ⅡA的含量

Determination of tanshinone Ⅱ A in Qishen granule by HPLC

目的建立芪参颗粒中丹参酮ⅡA的含量测定方法。方法采用HPLC法测定,色谱柱为AgiLent EcLipse XDB-C18柱(250 mm×4.6 mm,5μm),流动相为乙腈-0.2%乙酸梯度洗脱,流速为1.0 mL·min-1,测定波长为270 nm,柱温为20℃。结果丹参酮ⅡA的含量8.0～40.0 mg·L-1内与峰面积呈良好的线性关系,r=0.999 7,平均加样回收率为98.40%,RSD=1.03%(n=6)。结论该方法操作简便,结果准确,专属性强,可用于芪参颗粒的质量控制。

Objective To establish a method to determine the content of tanshinone ]I A in Qishen Granule. Methods tanshinone II A content was determined by HPLC. The column was AgiLent EcLipse XDB-Cms （250 mm ×4.6 mm, 5 μm） with the mobile phase composed of acetonitrile - 0.2% acetic acid gradient elution. The flow rate was 1.0 mL. min^- 1, the detection wavelength was 270 nm, the column temperature was 20℃. Results The good linear correiation existed in the range of 8.0 - 40.0 mg . L^-1 for tanshinone Ⅱ A （r = 0. 999 7）. The average recovery rate with added samples was 98.40%. The RSD was 1.03% （ n = 6）. Conclusions This method is simple, accurate and highly specific, which can be used for quality control of Qishen Granule.