樟芝发酵液不同萃取物体外生物活性

In vitro Biological ActivityAssociated with Extracts of Taiwanofungus camphoratus Spent Culture Broth

分别采用石油醚、氯仿、乙酸乙酯、正丁醇等有机溶剂对樟芝(Taiwanofungus camphoratus)发酵滤液进行萃取,萃取液在旋转蒸发仪上挥干,用少量的甲醇将其洗出自然挥干,进而研究樟芝发酵液不同萃取相的体外生物活性,结果表明:石油醚、氯仿、乙酸乙酯萃取物对人慢性粒细胞白血病细胞株K562的抑制作用明显,在5μg/mL(溶解于DMSO)的低浓度下即可达到60%以上(抑制率分别是74%、72%和66%),随着浓度的升高其作用高于5-Fu(抑制率68%);同样对于人前列腺癌LNCaP细胞,这三种萃取物的抑制作用也很明显,在25μg/mL下,三者的抑制率分别是72%、73%和69%,明显高于对照组5-Fu(抑制率66%);而对于RAW264.7巨噬细胞释放NO产量的刺激作用,只有正丁醇相在100、200、500μg/mL三种浓度下释放量分别达到12.12、13.75和14.32μmol/106 cells,明显高于对照组PBS(NO释放量为9.52μmol/106cells);对PC12神经细胞的修复作用,同损伤组相比,4种萃取物(除了25μg/mL浓度下正丁醇相)均有明显的功效,但是其作用浓度依赖性较小,在5μg/mL的低浓度下,几种萃取物的吸光度值分别是1.25、1.16、1.35和1.51,明显高于叠氮钠损伤组,显示了良好的修复作用。

Spent broth from submerged cultures of Taiwanofungus camphorata grown at 28 ~C for seven days was concentrated 10-fold under vacuum at 45 C, extracted with four different organic solvents （petroleum ether, chloroform, ethyl acetate and n-butanol） and, after removing the organic solvents in a rotary evaporator, the residues were dissolved in DMSO. Petroleum ether, chloroform and ethyl acetate extracts at 5 μg/mL concentration （in DMSO） inhibited the proliferation of K562 cells in vitro by 74%, 72% and 66%, respectively compared with 68% inhibition recorded with 100 μg/mL 5-flurouracil （5-Fu）. LNCaP cell proliferation was inhibited 72%, 73% and 69%, respectively by 25 μg/mL concentrations of petroleum ether, chloroform and ethyl acetate extracts compared with 66% inhibition by 100 μg/mL 5-Fu. NO production by RAW264.7 macrophages was 12.12, 13.75 and 14.32 μmol/106 cells following treatment with 100 μg/mL, 200 μg/mL and 500 μg/mL concentrations of n-butanol extract respectively, significantly higher compared to 9. 52 μmol NO/106 cells released following treatment with PBS. All the extracts （except 25 μg/mL n-butanol extract） exhibited a capacity to repair damage to PC12 cells induced by NAN3.