玉竹多糖的抗氧化作用研究

Antioxidant effect of polysaccharides in Polygonatum odoratum

为研究玉竹多糖的体外抗氧化活性和清除自由基作用,采用体外化学体系研究玉竹多糖对超氧阴离子(O-2·)、羟自由基(·OH)、1,1-二苯基-2苦苯肼自由基(DPPH·)和过氧化氢(H2O2)的清除能力。结果表明:玉竹多糖对超氧阴离子(O-2·)、过氧化氢(H2O2)和1,1-二苯基-2-苦苯肼自由基(DPPH·)具有较强的清除能力,其EC50值分别为0.38 mg/mL(Vc为0.09 mg/mL)、0.65 mg/mL(Vc为0.70 mg/mL)和0.43 mg/mL(Vc为0.06 mg/mL);但对羟自由基(·OH)的清除能力较弱,其EC50值为14.7 mg/mL(Vc为0.34 mg/mL)。玉竹多糖具有较好的体外抗氧化活性。

To achieve the effect of scavenging free radicals with polysaccharides in Polygonatum odora- turn, the scavenging ability of the polysaccharides on superoxide anion （ O2 · ）, hydroxyl radical （ · OH）, 1,1-dipheny 1-2-picrylhydrazyl （DPPH · ） and hydrogen peroxide （H202 ） ,sere studied by chemical systems in vitro. The polysaccharides from Polygonatum odoratum has good potential capacity for scavenging O2- · , DPPH· and H202 with EC50 values reaching 0.38, 0.43 and 0.65 mg/mL, respectively, and they are supe- rior to the scavenging · OH capacity, with EC50 values reaching 14.77 mg/mL. The polysaccharides of Po- lygonatum odoratum have stronger antioxidant effect in vitro.