呼气冷凝液中3种肿瘤标志物联合检测在肺癌诊断中的价值研究

Value of combined detection with three tumor markers in exhaled breath condensate on the diagnosis of lung cancer

目的对呼气冷凝液(EBC)中3种肿瘤标志物进行联合检测,研究其在肺癌诊断中的临床价值,探讨该方法在临床应用的可能性,为今后的肺癌诊断和治疗效果评估提供可靠的参考依据。方法选择2011年1月15日至2012年12月15日该院肿瘤科收治的肺癌患者30例作为肺癌组,另抽取同期健康体检者30例作为健康对照组,对肺癌组患者治疗前后和健康对照组进行EBC及血清中癌胚抗原(CEA)、细胞角蛋白19的可溶性片段(CYFRA21-1)、血管内皮生长因子(VEGF)水平检测,并对比分析检测结果。采用标准EBC收集器收集EBC,CEA、CYFRA21-1采用化学发光法,VEGF用酶联免疫吸附法测定。结果肺癌组患者EBC及血清中CEA、CYFRA21-1、VEGF检测水平明显高于对照组(P<0.05),化疗后CEA、CYFRA21-1、VEGF水平较治疗前显著降低(P<0.05),EBC中CEA、CYFRA21-1、VEGF水平较血清中低(P<0.05)。结论对EBC中CEA、CYFRA21-1、VEGF水平进行检测对于肺癌的诊断、病理分型和疗效判断均具有重要的参考价值,EBC检测结果与血清结果联合可实现相互补充,提高阳性率,值得关注。

Objective To combinedly detect three tumor markers in exhaled breath condensate, analyze the clinical value on diagnosis of lung cancer, discuss the possibility of the method applied in the clinic,and provide a reli- able basis for the lung cancer diagnosis and treatment in the future assessment. Methods 30 cases with lung cancer from January 15,2011 to December 15,2012 in our hospital were selected as lung cancer group, and the other 30 healthy people were selected as control group. The CEA,CYFRA21-1 and VEGF levels in exhaled breath condensate and serum were detected in lung cancer group and control group, and the detection results were comparatively ana- lyzed. Results The CEA,CYFRA21-1 and VEGF levels in lung cancer group were significantly higher than that of control group （P〈 0. 05）. CEA, CYFRA21-1 and VEGF levels were significantly decreased after treatment with chemotherapy than before（P〈0. 05）. In exhaled breath condensate, the CEA, CYFRA21-1 and VEGF levels were low than that in serum（P〈0.05）. Conclusion Detection of CEA,CYFRA21-1 and VEGF levels in exhaled breath condensate has important reference value in lung cancer diagnosis, pathological typing and curative effect judgment, and it is worthy of attention.