Th17和IL-17在肺炎支原体肺炎患儿中的检测意义

Significance of Thl7 and IL-17 in detecting mycoplasma pneumonia

目的探讨血清中白介素17（IL-17）以及外周血辅助性T细胞（Thl7）在肺炎支原体肺炎（MPP）患儿中的临床应用价值。方法选取确诊为肺炎支原体（MP）感染患儿268例为研究对象，同时选择100名健康儿童为对照组。分别采用ELISA法以及流式细胞术检测受试者血清中IL-17以及外周血Thl7的含量，对比分析不同临床症状患儿体内IL17、Thl7差异。结果MplgM阳性患儿体内IL-17、Thl7分别为（11．68±4．36）ng／L、（2．42±0．53）％，MplgM＋IgG阳性患儿体内IL-17、Thl7分别为（17．39±7．23）ng／L、（3．18±0．62）％，对照组IL-17、Thl7分别为（6．58±1．63）ng／L、（1．22±0．39）％，两两比较差异有统计学意义（P〈0．05）。不同临床表现的患儿体内IL-17、Thl7存在差异性。结论MPP的发病机制与IL一17水平以及Thl7频数异常有密切关系，两者可作为MPP患儿病情发展、转归的评价指标。

Objective To investigate the clinical value of serum interleukin-17 （IL 17） and peripheral blood T helper cells （Th17） in Mycoplasma pneumoniae pneumonia （MPP）. Methods 268 cases of Mycoplasma pneumoniae （MP） infection in children were selected for the study. 100 cases of healthy children were selected as the control group. IL-17 and Th17 in peripheral blood were detected by ELISA and flow cytometry. The IL 17 level and Th17 frequency of different positive antibodies children and children with different clinical symptoms were analyzed. Results IL-17 level and Th17 frequency were （11.68±4.36） ng/L and （2.42 ±0.53）% in positive MpIgM group, （17.39±7.23） ng/L and （3.18±0.62）% in positive MpIgM＋IgG group, （6.58±1.63） ng/L and （1.22±0.39）% in control group. The pairwise comparison difference was statistically significant （ P 〈0.05）. There was statistical significance in IL-17 and Th17 in children with different clinical manifestations. Conclusions The pathogenesis of MPP is closely related to IL-17 level and Th17 frequency, which can be used as the evaluation indexes of progression and outcome of MPP in children.