不同浓度碘对乳头状甲状腺癌B2-7细胞增殖和凋亡的影响

Effects of different concentration of iodine on the proliferation and apoptosis of papillary thyroid carcinoma B2-7 cell

目的 观察不同浓度碘对乳头状甲状腺癌B2-7细胞增殖和凋亡的影响.方法 体外培养乳头状甲状腺癌B2-7细胞,分为对照组和不同浓度碘干预组,使碘浓度分别为0,10-7,10-5,10-3 mol/L,干预24,72和120 h.四甲基偶氮唑蓝（MTT）法检测细胞增殖,AnnexinV-FITC/PI流式细胞术检测细胞凋亡,定量PCR检测凋亡抑制基因Bcl-2的表达.结果 MTT结果显示,与对照组相比,10-7mol/L组在72 h时吸光度值较高（P＜0.05）,但其在24 h和120 h时,以及10-5 mol/L组在3个干预时间差异均无统计学意义（P＞0.05）.而10-3 mol/L组在3个干预时间吸光度值均低于对照组（P＜0.05）.与对照组相比,24 h时各干预组细胞凋亡率差异无统计学意义（P均＞0.05）,而72 h时10-3 mol/L组细胞凋亡率升高（P＜0.05）,10-7、10-5mol/L组差异均无统计学意义（P＞0.05）.120 h时各干预组细胞凋亡率均高于对照组（P均＜0.05）.与对照组相比,24 h时各干预组凋亡抑制基因Bcl-2表达水平差异无统计学意义（P＞0.05）,72 h时10-3 mol/L组Bcl-2表达水平下调（P＜0.05）,10-5和10-7mol/L组差异均无统计学意义（P均＞0.05）,120 h时10-3 mol/L组表达水平仍下调,而10-7mol/L组却高于对照组（P＜0.05）.结论 体外高浓度碘对乳头状甲状腺癌B2-7细胞有抑制增殖和促凋亡作用.

Objective To observe the effects of different concentration of iodine on the prolifera-tion and apoptosis of papillary thyroid carcinoma B2-7 cell.Methods Papillary thyroid carcinoma B2-7 cells were cultured in vitro and divided into control group and different concentration kalium iodide intervene groups,concentration of iodine were 0,10-7,10-5,10-3 mol/L,respectively,the intervening time were 24,72 and 120 h.The cell proliferation was measured by methyl thiazolyl tetrazolium （MTT） assay.The cell apoptosis was analyzed by AnnexinV-FITC/PI flow cytometry assay and expression of apoptosis suppressor Bcl-2 was detected by quantitive RT-PCR.Results MTT assay showed the absorbance value of 10-7 mol/L group at 72 h was higher （P 〈 0.05）,and there were no statistical differences at 24,120 h compared with control group,as well as 10-5 mol/L group at intervention time 24,72 and 120 h （P 〉0.05）.While absorbance values of 10-3 mol/L group at three intervention time were lower than those of control group （P 〈 0.05）.The ratio of apoptosis cells among intervention groups had no statistical difference at 24 h compared with control group （all P〉0.05）.At 72 h,the ratio of apoptosis cells in 10-3 mol/L group increased （P 〈0.05）,while in 10-7,10-5mol/L groups,the differences had no statistical significance （P 〉0.05）.At 120 h,the ratio of apoptosis cells in each intervention groups were all higher than those in control group （P 〈0.05）.There were no statistical differences in the expression of Bcl-2 among intervention groups at 24 h compared with control group （P 〉 0.05）.However,its expression in 10-3 mol/L group was lower than that of control group at 72 h （P 〈 0.05）,and had no statistical differences in 10-5,10-7 mol/L groups （all P 〉0.05）.At 120 h,the expression of Bcl-2 was down-regulated in 10-3 mol/L group and up-regulated in 10-7 mol/L group.Conclusion High iodine could inhibit proliferation of papillary thyroid carcinoma B2-7 cell and promote its apoptosis in vitro.