胃癌腹膜种植瘤动物模型的构建

Establishing a peritoneal dissemination xenograft mouse model for human gastric cancer

目的建立稳定实用的人胃癌鼠腹膜种植瘤模型。方法通过对裸鼠和SCID鼠腹腔注射胃癌细胞株AGS、NCI—N87和SNUl6构建腹膜种植瘤模型，比较不同细胞株模型成功率和生存期的差异。结果裸鼠腹腔种植5×106个AGS、NCI—N87和SNUl6细胞腹膜瘤形成率分别是（3／8、6／8和2／8；SCID鼠腹腔种植5×106个AGS、NCI—N87和SNUl6细胞，腹膜瘤形成率分别是0／6、6／6和6／6。SCID鼠腹膜瘤模型中位生存期：NCI—N87细胞组（10x10。）为74d；SNUl6细胞3个不同剂量组（10×106、20×106和40×106）分别为95、78和44d。结论SCID鼠腹腔种植（20—40）×106个SNUl6细胞可以构建稳定实用的胃癌腹膜种植瘤模型。

Objective To establish a stable and convenient peritoneal dissemination mouse model for experimental gastric cancer research. Methods Human gastric cancer cell lines AGS, NCI-N87 and SNU-16 were intraperitoneally injected into nude mice and SCID mice. The peritoneal tumor formation and mouse survival were compared among different groups. Results The formation rates of peritoneal tumor in nude mice by intraperitoneal injection of 5 x 106 AGS, NCI-N87 and SNU16 cells were 0/8, 6/8 and 2/8, respectively. The formation rates of peritoneal cancer in SCID mice after implanting 5 x 10s cells were 0/6 for AGS cell line, 6/6 for NCI-N87 and 6/6 for SNU16. The median survival time in SCID mice was 74 days after intraperitoneal injection of 10 × 106 NCI-N87 cells, 95 days for 10× 106 SNU16 cells, 78 days for 20×106 SNU16 ceils, and 44 days for 40×106 SNU16 ceils, respectively. Conclusions A peritoneal dissemination mouse model is successfully established by intraperitoneal injection of （20-40）× 106 SNU16 cells. It is a reliable and feasible model for gastric cancer research.