摘要
对家蚕线粒体基因组EcoRⅠ 2 .0kb片段进行了克隆和序列分析 ,结果表明该片段包含完整的丝氨酸转移RNA基因、NADH氧化还原酶亚基Ⅰ基因、细胞色素氧化酶b亚基基因的部分序列。与果蝇 (Drosophilamelanogaster)的mtDNA进行了同源性比较 ,并根据无脊椎动物线粒体基因组密码子表 ,推定氨基酸序列。
In this study, Eco RⅠ2.0kb fragment of mitochondrial genome DNA(mtDNA) of silkworm was cloned and sequenced.The results showed that this cloned fragment contains the complete gene of tRNA Ser,partial sequences of oxido reductas NADH subunit I gene and cytochrome oxidase subunit b gene.The homologies of these genes were compared with that of fruitfly, Drosophila melanogaster mtDNA.The amino acid sequences were deduced according to the mitochondrial codon of invertebrate as well.
出处
《蚕业科学》
CAS
CSCD
2000年第4期234-238,共5页
ACTA SERICOLOGICA SINICA
