鸭疫里默菌的分离鉴定及免疫原性试验

Identification and Immunogenicity Test of R.anatipestifer Isolated from a Duck Farm

2008年-2012年，对北京某鸭场送检的鸭传染性浆膜炎疑似病例进行了鸭疫里默菌的分离鉴定，通过病原菌的分离培养、涂片镜检、生理生化试验对该病做出初步诊断，并进一步进行了其血清型鉴定、致病性试验和免疫原性分析。结果表明，成功分离到11株细菌，并可以分为3个血清型，以1型和2型感染为主；攻毒试验表明11株分离株对14日龄樱桃谷雏鸭致病性差异较大，从40％～100％不等；免疫试验结果表明分离株JX-2、JX-6和JX-10制成的疫苗攻毒保护率均可以达到80％以，应考虑使用含此3种血清型的菌株制成多价苗对该养殖场进行预防接种。

The ducks collected from a duck farm in Beijing during 2008 -2012 were suspected of suffering from infectious serositis .The R .anatipesti f er isolates were isolated from the ducks .The disease was diag-nosed preliminary through pathogen isolation ,biochemical test ,and the serotype identification ,pathoge-nicity and immunogenicity were also conducted .Results indicated that ,11 isolates isolated from the ducks were divided into three serotypes ,mainly serotype 1 and 2 .The pathogenicity test showed that pathoge-nicities of 11 isolates on 14 day-old Cherry Valley duckling varied greatly ,ranging from 40% to 100% . Immunogenic test results showed that the protective rate of the vaccines made of the strains JX-2 ,JX-6 and JX-10 reached more than 80% .The polyvalent vaccine could be made of the strains of 3 serotypes and vac-cinated in the farm .