成年维生素D缺乏大鼠模型的建立及评价

Establishment and evaluation of model for adult rats with vitamin D deficient

目的建立维生素D缺乏大鼠模型,并进行初步评价,为研究维生素D缺乏与高血压的关系奠定基础。方法将成年的SD大鼠分成正常对照组、维生素D缺乏组和维生素D对照组,分别采用普通正常饲料、维生素D缺乏饲料和维生素D对照饲料进行饲养,饲养环境采用白炽灯作为照明光源,维持12h:12h光暗周期;饲养6周后通过眼底取血的方式采集外周血,使用EIA法测定血清维生素D含量,之后每隔两周测定1次。结果饲养10周后维生素D缺乏组大鼠血浆维生素D平均含量降至2.07nmol/L;维生素D对照组为56.74nmol/L,正常对照组为57.61nmol/L,正常对照组与维生素D对照组差异无统计学意义(P>0.05)。与其余两组相比,维生素D缺乏组大鼠血压显著升高。结论通过控制光照和特殊饲料饲养方法建立维生素D缺乏大鼠模型是可行的,相比对照组,维生素D缺乏动物的血压显著升高。

Objective To establish and evaluate animal model of adult rats with vitamin D deficient and assess the association of vitamin D deficiency and hypertension. Methods Adult SD rats were divided into 3 groups：normal controls group（feed with ordinary forage） , vitamin D deficient groups（feed without vitamin D）and vitamin D control group（feed with vitamin D）, which were fed with different diets under t2 h： 12 h without ultraviolet light bulb（UV-B）. After treatment for 6 weeks,Retinal blood was collect- ed,and Serum vitamin D levels was tested by EIA every 2 weeks. Results After treatment for 10 weeks,Serum level of vitamin D in vitamin D deficient group were reduced to 2.07 nmol/L,while vitamin D group and normal control group were measured as 56.74 nmol/L and 57.61 nmol/L,there was no statistically significant difference between vitamin D group and normal control group（P〈 0.05）. hypertension of vitamin D deficient group was significantly higher than other two groups. Conclusion It is feasible to establish model of adult rats with vitamin D by UV-B free light and diets control,hypertension of rats vitamin D deficiency has a signifi- cant rise compared with the control group.