复苏液体对重度失血性休克大鼠心肌热休克蛋白90α表达的影响

Expression changes of HSP90α in cardiac muscles in rats with severs hemorrhagic shock by the treatment of different resuscitating fluid

目的用不同种类液体对重度失血性休克大鼠进行复苏后,对比大鼠心肌组织中热休克蛋白(HSP)90α的表达、变化规律及大鼠生存率,为临床治疗失血性休克选择复苏液体提供参考。方法建立失血性休克大鼠模型,分别运用乳酸林格液、聚明胶肽注射液、高渗氯化钠右旋糖酐进行液体复苏,测定大鼠心肌组织中HSP90α蛋白的表达及大鼠死亡率。结果重度失血性休克后运用不同种类液体进行复苏,大鼠心肌组织HSP90α表达、大鼠死亡率差异有统计学意义(P<0.05)。结论重度失血性休克可以诱导大鼠心肌组织中HSP90α的表达,当用不同液体进行复苏后各组HSP90α的表达不同且具有一定的规律性,提示HSP90α作为一个重要的调控分子参与失血性休克后心肌组织的保护机制。

Objective To explore the expression changes of HsPgα in cardiac muscles and survival rates in rats by using the different fluids to resuscitate the severs hemorrhagic shocked rats, and provide reference for the clinical treatment of hemorrhagic shock with different resuscitation fluids. Methods Uncontrolled hemorrhagic shock rats model was established, using lactic acid salinger liquid, poly peptide injection gelatin, hypertonic sodium chloride dextran for fluid resuscitation respectively, and then checked the HSP90α expression changes and survival rates in rats. Results the expressions of HSP90α in myocardial tissue and the mortality in rats were different after using different resuscitation fluids in severe hemorrhagic shock rats, difference was statistically significant（P〈0.05）. Conclusion the expression of HSP90α in cardiac muscles of rats could be induced by severe hemorrhagic shock,the HSP90α expressed differently and regularly after using different resuscitating fluids,it implied that the HSP90α played an imoortant role in the hemorrhagic rats cardiac as a regulating fator.