微波-氯化锂复合诱变筛选耐受高浓度丁醇菌株

Screening of High Concentration Butanol Tolerance Strains by Microwave-LiCl Complex Mutation

菌株耐受丁醇能力不高是制约提高传统丁醇发酵产量的重要因素之一,选择并构建新的丁醇生产异源重组菌株并提高其耐受高浓度丁醇的能力是提高丁醇能力的有效途径。试验以丁醇耐受性为2.5%的发酵乳杆菌为出发菌株,对其进行微波-氯化锂复合诱变处理,然后经过初筛和复筛,得到丁醇耐受性提高的菌株。结果表明:当微波功率为800 W,频率为2 450 MHz,微波处理时间为55 s,氯化锂浓度为1.2%时,致死率达85.96%,突变菌株丁醇耐受性提高到3%,并且遗传稳定性实验证明其丁醇耐受性遗传稳定。

One of the important factors to restrict traditional production of butanol fermentation was the low ability of strains tolerance butanol.It could effectively improve the butanol capacity by selecting and constructing new heterologous recombinant strains of butanol production and improving its ability to tolerate high concentrations butanol.In this experiment,the Lactobacillus fermentum with butanol tolerance of 2.5% as the original strain was conducted through microwave - LiCl complex mutation,screened and rescreened,then the strains with high ability of tolerance butanol were obtained. The results showed that under the condition of 800 W microwave power,2 450 MHz frequency,55 s microwave treatment, 1.2% concentration of LiC1 and 85.96% death rate,the ability butanol tolerance of mutant strains was increased to 3%, and their heredity stability was proved by genetic stability experiments.