贵州省都匀市茶轮斑病病原菌鉴定及生物学特性的研究

The Identification of Tea Gray Blight Pathogen and Study of Biological Characters in Duyun City,Guizhou Province

对贵州省都匀市出现的茶轮斑病的症状进行了系统的描述,采用致病性试验、形态学观察及分子生物学的方法对茶轮斑病的病原菌进行鉴定。致病性试验证实,不同的接种方法对茶叶的致病力均有不同。对病原菌rDNA-ITS区进行了PCR扩增和序列测定。综合形态特征、致病力和分子序列分析结果,确定茶轮斑病病原菌为茶拟盘多毛孢菌(Pestalotiopsis theae)。

This study detailedly described the symptom of tea gray blight in Duyun city, Guizhou Province. The pathogenic test, morphological observation and molecular method were used for identification of pathogenic fungus. The pathogenic test confirmed that different inoculation methods influenced the pathogenicity. It also amplified the rDNA-ITS by PCR reaction and sequenced it. Integrated morphological characters, pathogenicity and molecular analysis, it is concluded that the pathogen of tea gray blight was Pestalotiopsis theae.