H9亚型禽流感病毒感染引起MDCK细胞内抗氧化功能的变化

Effect of Avian Influenza Virus Subtype H9 on Antioxidant Function in MDCK Cells

将传代MDCK细胞以8×104/cm2的密度接种于6孔细胞培养板,H9亚型禽流感病毒以不同剂量0(对照组)、10-3(高剂量组)、10-4(中剂量组)、10-5×EID50(低剂量组)分别接种,检测H9亚型禽流感病毒对MDCK细胞内抗氧化功能的影响。结果表明,与对照组相比,高剂量H9亚型禽流感病毒接种MDCK细胞使细胞内过氧化氢(H2O2)和羟基自由基(·OH)含量均显著增加(P>0.05),细胞内超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)活力均显著下降(P>0.05),细胞内的丙二醛(MDA)含量显著增加(P>0.05)。说明H9亚型禽流感病毒可显著提高MDCK细胞内活性氧自由基(ROS)含量,并降低抗氧化酶活力,阻碍ROS在细胞内的清除机制,引起细胞脂质过氧化作用,从而损伤细胞。

After MDCK cells were seeded in 6-well culture plate at a density of 8 × 104/cm^2, avian influenza virus subtype H9 were inoculated with those cells at different doses （0,10-3 × EID50,10-4 ×EID50 and 10^-5 × EID50 ）. Experimental results showed that compared with the control group, hydrogen peroxide （H2 02 ） and hydroxyl radical （ · OH） in MDCK cells inocu- lated high-dose of avian influenza virus subtype H9 enhanced significantly （P〉0.05）, and superoxide dismutase （SOD） and glutathione peroxidase （GSH-Px） activity significantly decreased （P〉0.05） in those cells. In addition,intracellular malondial- debyde （MDA） had a significant increasing （P〉0.05） in MDCK cells treated with high-dose of virus. In conclusion,avian in- fluenza virus subtype H9 could significantly enhance the content of reactive oxygen species （ROS） in MDCK cells, reduce the activities of antioxidant enzymes, and hinder ROS scavenging mechanisms within the cell, cause the cell lipid peroxidation, which damaged cells.