摘要
用保加利亚乳杆菌制备L-谷氨酸脱羧酶(GAD)粗提物,通过化学消旋由L-谷氨酸制备DL-谷氨酸,用GAD催化DL-谷氨酸中的L-谷氨酸生成γ-氨基丁酸,利用等电点沉淀法获得D-谷氨酸和γ-氨基丁酸。
DL-Glutamic acid was transformed from L-glutamic acid by the chemical racemizing. L-Glutamic acid generated γ-aminobutyric acid by decarboxylic reaction with the catalysis of crude extract of L-glutamic acid decarboxylase from cells of Lactobacillus delbrueckii sp. Bulgaricus. When the reaction finished, L-glutamic acid had been selectively consumed and left D-glutamic acid and γ-aminobutyrie acid in reaction system.
出处
《化学与生物工程》
CAS
2013年第11期55-56,共2页
Chemistry & Bioengineering
关键词
L-谷氨酸脱羧酶
D-谷氨酸
Γ-氨基丁酸
L-glutamic acid decarboxylase
D-glutamic acidlγ -aminobutyric a＇cid（GABA）
