DNMT3B基因启动子单核苷酸多态性与结直肠癌易感性的关联研究

Correlation between polymorphism in the promoter of DNA methyltrausferase-3B gene and susceptibility of coloreetai cancer

目的通过对天津地区汉族结直肠癌（cRc）患者和正常人群中DNA甲基转移酶3B（DNMT3B）基因单核苷酸多态性（SNP）位点rsl569686基因型的检测，探讨rsl569686位点基因型与CRC易感性的关系。方法应用Sequenom MassArray飞行时间质谱系统检测89例CRC患者（观察组）及94例健康者（对照组）DNMT3B基因多态位点rsl569686的基因分型，判断其与CRC的相关性。结果DNMT3B基因多态位点rsl569686的3种基因型Iq＂、GT、GG在CRC患者中的频率分别为74．2％、24．7％、1．1％，与对照组（73．4％、23．4％、3．2％）相比，差异无统计学意义（P〉O．05）。结论天津汉族人群CRC的遗传易感性可能与DNMT3B基因rsl569686位点的SNP无关。

Objective To observe and analyze the correlation between the DNA methyltransierase-3B （DNMT3B） gene single nucleotide polymorphisms （SNP） rs1569686 and the susceptibility to colorectal cancer （CRC） in Tianjin Ylan population. Methods A case-control study was performed. 89 CRC patients and 94 normal controls were examined, and Sequenom MassArray system was applied to detect the genotype of rs1569686 in DNMT3B gene polymorphisms. The association results were analyzed of the SNP and CRC. Results The frequencies of genotypes TF, GT and GG at SNP rs1569686 were 74.2%, 24.7% and 1.1% in CRC patients respectively, which there were no significant differences from that in controls（73.4%,23.4% and 3.2%; P〉0.05）. Conclusion It suggests that the SNP rs1569686 of DNMT3B gene may not be related to susceptibility of CRC in Tianjin Han population.