高效液相色谱-质谱联用法测定人血浆中麦考酚酸的浓度

Determination the concentration of mycophenolic acid in human plasma by HPLC-MS

目的建立高效液相色谱-质谱联用测定人血浆中麦考酚酸浓度的方法。方法以苯巴比妥为内标，色谱柱为ZORBAX Eclipse XDB—C18（2．1mm×150mm，3．5μm），流动相为0．05％乙酸铵-乙腈（45：55），流速为0．2mL·min^-1，柱温35℃，电喷雾离子源（ESI）正负离子同时检测。结果麦考酚酸的线性范围为0．5—100．0μg·mL^-1；最低检测限分别为0．1μg·mL^-1。高、中、低3个浓度的日内标准偏差小于3％，日间标准偏差小于15％，绝对回收率均大于70％。结论本方法简便、快速、灵敏、重现性好，能有效检测人血浆中麦考酚酸的含量。

Objective To establish a HPLC -MS method for determina- tion the concentration of mycophenolic acid in human plasma. Methods Phenobarbital was used as internal standard. After precipitation, the analysis was chromatographed on a XDB - C18 （2. 1 mm ×150mm,3.5μm）. An isocratic mobile phase consisted of 0. 05% ammonium acetate in water - aceto- nitrile （45：55）. The flow rate was 0. 2 mL· min^-1 and column temperature was 35 ℃. ESI electrospmy ionization source in both negative and positive modes. Results The calibration curves of each drug showed a good linearity in a range of concentration between 0. 5 - 100. 0 μg·mL^-1 for sodium valproate. Intra and inter precision（RSD% ） were less than 3% and 15% , respectively. The recoveries were above 70%. Conclusion The method is simple, rapid, sensitive and stable which is suitable for determination of the concentration of mycophenolic acid in human plasma.