华山松松节的化学成分及黄嘌呤氧化酶抑制活性研究

Studies on the constituents of Pinus armandii with xanthine oxidase inhibitory activities

目的研究华山松松节的化学成分和黄嘌呤氧化酶抑制活性。方法通过各种柱色谱方法分离化合物,通过理化性质和波谱数据进行结构鉴定,对提取物和分离得到的化合物通过紫外分光光度法测定黄嘌呤氧化酶抑制活性。结果从松节提取物的石油醚部分共分离得到8个化合物,分别鉴定为异海松-7-烯-18-酸(1),唐松酸(2),3β,21α-二甲氧基锯齿石松-14-烯(3),3β-甲氧基-21α-乙酰氧基-锯齿石松-14-烯(4),3-甲氧基-锯齿石松-14-烯-21-酮(5),β-谷甾醇(6),胆甾醇肉豆蔻酸酯(7)和山嵛酸(8)。结论化合物3为首次从该植物中分离得到,并首次报道了该化合物的碳谱数据;化合物1和2具有一定的黄嘌呤氧化酶抑制作用。

Objective To study the chemical constituents of the knobs of Pinus armandii and their xanthine oxidase inhibitory ac-tivities .Methods Compounds were isolated and purified by using various chromatographic technologies and their structures were identified on the basis of physicochemical properties and spectroscopic data .The xanthine oxidase inhibitory activities were deter-mined by UV spectrophotometric method .Results Eight compounds were identified as isopimara-7-en-18-oic acid （1） ,lambertian-ic acid （2） ,3β,21α-dimethoxyserrat-14-ene （3） ,3β-methoxyl-21α-acetoxyserrat-14-ene （4） ,3β-methoxyserrat-14-en-21-one （5） ,β-sitosterol （6） ,cholesteryl myristate （7） and docosanoic acid （8） .Conclusions Compound 3 was isolated from this plant for the first time ,and also its 13C-NMR data were reported for the first time .Compound 1 and 2 showed some xanthine oxidase inhibitory activities .