紫外-可见分光光度法测定曲安奈德尿素乳膏中醋酸曲安奈德的含量

Content Determination of Triamcinolone Acetonide in Triamcinolone Urea Cream by UV-visible Spectrophotometry

目的：建立测定曲安奈德尿素乳膏中醋酸曲安奈德含量的方法。方法：采用紫外。可见分光光度法。以乙醇为溶剂，氯化三苯四氮唑、氢氧化四甲基铵为反应试液，在485nm波长处测定。结果：醋酸曲安奈德检测质量浓度线性范围为4．992~22．464gg／ml（r=0．9999），平均加样回收率为100．21％（RSD=0．63％，n=9）。结论：建立的方法简便、灵敏、准确，可用于该制剂的质量控制，、

OBJECTIVE： To establish a method for the content determination of triamcinolone acetonide in Triamcinolone urea cream. METHODS： UV-visible spectrophotometry was adopted. Using triphenyltetrazolium chloride and tetramethylammonium hy- droxide as reaction solution, ethanol as solvent, triamcinolone acetonide was determined at 485 nm. RESULTS： The linear range of triamcinolone acetonide was 4.992-22.464 gg/ml （r=0.999 9） with an average recovery of 100.21% （RSD=0.63% , n=9）. CONCLUSIONS： The method is simple, sensitive and accurate, and it is suitable for quality control of the preparation.