青海湖裸鲤核糖体蛋白S3a基因的克隆和表达特性研究

Cloning and characterization of ribosomal protein S3a mRNA from Gymnocypris przewalskii

青海湖裸鲤是青海湖及其周边流域唯一的经济鱼类,在鱼-鸟-草地生态系统中处于核心地位。核糖体蛋白S3a(RPS3a)是真核细胞中核糖体40S小亚基的组成成分,为一种多功能性蛋白。开展青海湖裸鲤高盐适应机理的研究,有助于揭示青海湖裸鲤的基本生命活动规律,为该鱼种的资源保护和人工增殖放流提供理论依据。我们通过RT-PCR和RACE技术,得到了青海湖裸鲤RPS3a的完整编码序列[KC818610.1]。通过实时荧光定量检测胚胎发育不同阶段和不同盐度胁迫条件下RPS3a表达水平的变化,显示其在盐度胁迫条件下和胚胎发育过程中表达水平逐步上升,初步表明RPS3a对于青海湖裸鲤胚胎发育和盐度适应性生理机制的变化有着重要作用。

Gymnocypris przewalskii is the only kind of economic fishes distributed in Qinghai Lake located on the Qinghai Tibet plateau. Ribosomal protein S3a （RPS3a） is one kind of multifunctional protein known as a component of the 40s small ribosomal subunit. Research concerning molecular mechanism of Gymnocypris przewalskii  under salinity stress will contribute to discovering the basic pattern of vital activity and provide theories for conservation and artificial reproduction of this species. In this study, the cDNA sequences of RPS3a [KC818610.1] were obtained from the naked carp through reverse transcriptase polymerase chain reaction （RT-PCR） and 5′ and 3′ rapid amplification of cDNA ends （RACE-PCR）. According to real time PCR analysis, the transcriptions of RPS3a increased in embryogenesis and under salinity stress, and it was demonstrated that RPS3a played an important role in embryogenesis and salinity adaptation.