^(252)Cf裂变中子辐照豌豆种子生物学效应的初步研究

Preliminary study on biological effects of pea seeds(Pisum sativum L.) induced by ^(252)Cf neutron source

以豌豆干种子为研究对象,采用252Cf裂变中子源对其辐照。利用蒙特卡罗(Monte Carlo,MCNP)程序分别模拟了中子及γ射线的输运,计算了不同辐照位置样品的中子吸收剂量和γ射线(光子)吸收剂量。研究了辐照后不同吸收剂量引起的生物学效应,与对照组的比较表明:低吸收剂量(0.239-4.330 Gy)的中子辐照豌豆干种子后,M1代豌豆进入花期的时间延迟,微剂量的中子辐照促使M1代豌豆出苗率升高;中子辐照能够促进M1代豌豆苗期分支增多;适当剂量(0.619 Gy)的中子辐照能够显著提高M1代豌豆的收获量。

The doses and biological effects of irradiated plants have been studied and reported widely. In the research, Co-60 gamma-ray source was more often used than the neutron source. However, fast neutron source is promising in such irradiation studies as it has many advantages, such as strong biological effect, high mutation rate, wide variation spectrum and stable mutant. Purpose： We aim to explore the method of dose calculation in pea samples. The biological effects of pea seeds （Pisum sativum L.） induced by different radioactive doses are to be investigated. Methods： The Needle Leaf Pea seeds were irradiated using 252Cf fission neutron source. The Monte Carlo simulation MCNP4C code was used to calculate the neutron absorbed doses and y-rays （photons） absorbed doses in the samples. The biological effects of pea seeds induced by different radioactive doses were investigated. Results： The results showed that the flowering time of M1 generation peas was delayed with lower neutron absorbed doses （0.239-4.330 Gy）, and the germination rate was promoted with micro-absorbed doses. The seedling branch rate of M1 generation peas was elevated by neutron radiation. The harvest of M1 generation was increased with appropriate neutron doses （0.619 Gy） irradiating peas. Conclusion： After being irradiated by low neutron doses （0.239-4.330 Gy）, M1 generation peas show obvious biological effects. The research results have positive significance for the agricultural production and breeding.