摘要
以卫星搭载筛选出的2株突变株系为材料,研究空间环境对苜蓿(Medicago sativa)蛋白表达机制的影响,利用IEF/SDS-PAGE凝胶电泳技术,对其蛋白质特异性进行了比较分析。结果表明,突变株系与对照相比,蛋白质图谱有较大的差异,突变株系1L-4较对照共有6个蛋白质斑点下调,17个蛋白质斑点上调,突变株系6L-5较对照共有17个斑点下调,18个斑点上调,表明空间环境促使了苜蓿基因结构的改变,影响了蛋白质的表达。
Taking two alfalfa（Medicago sativa ） mutant strains selected by satellite launch as materials. effects of spaceflight on protein expression mechanism of alfalfa mutant were studied through IEF/SDS- PAGE gel electrophoresis. And a comparative analysis on protein specificity was conducted. The results showed that there are huge difference between protein maps of mutant strain and control. For mutant IL- 4, six protein spots are lower than those of control, and 16 protein spots are higher than those of control. For mutant 6L-5, 17 protein spots are lower than those of control and 18 protein spots are higher than those of control. This indicated that space environment causes the changes on genetic structure of alfalfa, and then affects i
出处
《草业科学》
CAS
CSCD
北大核心
2013年第11期1749-1754,共6页
Pratacultural Science
基金
国家科技支撑计划项目(2011BAD17B01)
国家科技支撑计划项目(2012BAD12B00)
黑龙江省教育厅科学技术研究项目(12521610)
关键词
卫星搭载
苜蓿
差异蛋白质
双向电泳
space ts protein expression. flight
alfalfa
differential proteins
2D-PAGE
